TABLE 3.
Distribution of muropeptides in B. subtilis HR (wild type) at different growth phases and in AA106 (ponA) and AA109 (dacA) in late stationary phase
| Strain | Growth phase (h) | % Muropeptidesa
|
% Cross-linking
|
||||
|---|---|---|---|---|---|---|---|
| Monomers | Dimers | Trimers | Tetramers | RP-HPLC | Gel filtration | ||
| HR (wild type) | Exponential (2) | 43.6 | 50.3 | 5.8 | 0.2 | 29.1 | 39.6 |
| Late exponential (4) | 39.5 | 53.4 | 6.8 | 0.3 | 31.4 | 41.4 | |
| Stationary (6) | 37.2 | 54.3 | 8.1 | 0.3 | 32.8 | 43.0 | |
| Late stationary (8) | 36.1 | 54.8 | 8.4 | 0.3 | 33.2 | 44.0 | |
| AA106 (ponA) | Late stationary (8) | 47.4 | 49.4 | 2.8 | 0 | 26.6 | NDb |
| AA109 (dacA) | Late stationary (8) | 36.0 | 58.8 | 4.5 | 0.2 | 32.5 | ND |
a
Determined from RP-HPLC-resolved muropeptides.
b
ND, not determined.