Table 4.
List of antibodies used in cell biology studies with experimental dilutions
| Antibody | Catalogue number | Clone | Company | Dilution used |
|---|---|---|---|---|
| Anti-eIF4E (phospho S209) | AB76256 | EP2151Y | Abcam | 1:5000 |
| Anti phospho 4EBP1 threonine 36/46 | 2855 | 236B4 | Cell signalling Technology | 1:1000 |
| Anti p44/42 MAPK | 9102 | Not provided | Cell signalling Technology | 1:1000 |
| Anti pAKT ser473 | 9271 | Not provided | Cell signalling Technology | 1:1000 |
| Anti eIF4E | 2067 | C46H6) | Cell signalling Technology | 1:500 |
| Anti 4EBP1 | 9644 | 53H11 | Cell signalling Technology | 1:4000 |
| Anti phospho p44/42 | Not provided | Cell signalling Technology | 1:1000 | |
| Anti AKT | Not provided | Cell signalling Technology | 1:1000 | |
| Anti eiF4G1 | 2858 | Not provided | Cell signalling Technology | 1:500 |
| Anti cyclin D1 | 2988 | 92G2 | Cell signalling Technology | 1:500 |
| Anti Mcl-1 | 5453 | D35A5 | Cell signalling Technology | 1:1000 |
| Anti 4EBP2 | 2845 | Not provided | Cell signalling Technology | 1:1000 |
| Anti Bcl-Xl | 2764 | 54H6 | Cell signalling Technology | 1:1000 |
| Anti Parp | 9532 | 46D11 | Cell signalling Technology | 1:1000 |
| Anti Stat-1 | 9172 | Not provided | Cell signalling Technology | 1:1000 |
| Anti β-actin peroxidase | A3854 | AC-15 | Sigma aldrich | 1:10,000 |
| Anti-FLAG peroxidase | A8592 | M2 | Sigma aldrich | 1: 1000 |
| Anti Phospho-S6 Ribosomal subunit Ser235/236 | 2211 | Not provided | Cell signalling Technology | 1: 1000 |
| Anti S6 Ribosomal subunit | 2317 | 54D2 | Cell signalling Technology | 1: 1000 |