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Histidine imidazole side-chain signals upon interaction with MHET. Selected region from the 1H-13C HMQC spectrum of LCC-CC (A), LCC-ICC (B), and LCC-ICCG (C) at 60°C in the absence of MHET (blue) and in the presence of 4 mM (orange) and 10 mM (red) MHET. (D) 1Hε-13Cε combined CSPs induced by 10 mM MHET for the histidines of LCC-CC (blue), LCC-ICC (gray), and LCC-ICCG (violet). To see this figure in color, go online.
