Figure 7.

Continuum mechanics-based FRAP onto mClover2-actin in stress fibers of A7r5 cells treated with 10-nM Latrunculin A. (A) Pre-bleach image. (B) The displacement vectors. Color bar represents the angle of the displacement vectors. Insets of the indicated regions are magnified 3×. (C) The values of strain parallel with stress fibers in the reference frame are overlaid in the cell image. (D) The actin molecular turnover results in no correlation with the magnitude of velocity parallel with stress fibers ($R\left(k,\left|v_{\parallel }\right|\right)=-0.17$; $p=0.059$; $n$ = 119 from independent $N$ = 3 cells). (E) The actin molecular turnover is not correlated with the magnitude of strain parallel with stress fibers ($R\left(k,\left|{\epsilon }_{\parallel }\right|\right)=0.19$; $p=0.45$; $n$ = 119 from independent $N$ = 3 cells). Lines represent the regression fitting for variables of S1 and S5 (blue), S2 and S4 (green), and S3 (red), where the definition of S1–S5 is shown in Fig. 5A. (F) Model of chemomechanical behavior of stress fibers in cells. To see this figure in color, go online.