Figure 6.

Experimental observations of clathrin-coated pits within CH27 B cells, imaged using a nanobody-coupled Atto655 DNA-PAINT scheme. (a) (Left) Reconstructed image (16 nm pixels, 20 nm Gaussian blur) with yellow dashed line indicating the region of interest interrogated. (Right) A magnified subset from the white square region of larger image (1 nm pixels, 4 nm Gaussian blur) along with a scatterplot of localizations with color representing the observation time. Scale bars, 2 μm (left) and 200 nm (right top and bottom). (b) Autocorrelations as a function of displacement $g(r,\tau )$, tabulated from localizations for time interval windows centered at the values shown. (c) $\text{Δ}g\left(r,\tau \right)=g\left(r,\tau \right)-g\left(r,{\tau }_{\max }=274s\right)$ for the examples shown in (b). (d) $\Delta g(r,\tau )$ are fit to $\text{Δ}g\left(r,\tau \right)\propto \exp \left\{-r^2/4{\sigma }_{xy}^2\right\}$ to extract the LSF width in each lateral dimension. Error bars represent estimates of the standard error obtained through bootstrapping. The average resolution for this image is $⟨{\sigma }_{xy}⟩=$ 11.6 ± 0.3 nm. To see this figure in color, go online.