Figure 7.

Experimental observations of F-actin on the ventral surface of a CH27 B cell using phalloidin-AlexaFluor647. (a) (Left) Reconstructed image (50 nm pixels, 50 nm Gaussian blur) with yellow dashed line indicating the region of interest interrogated. (Right) A magnified subset from the white square region of larger image (1 nm pixels, 10 nm Gaussian blur) along with a scatterplot of localizations with color representing the observation time. Scale bars, 5 μm (left) and 500 nm (right top and bottom). (b) Autocorrelations as a function of displacement $g(r,\tau )$, tabulated from localizations for time interval windows centered at the values shown. (c) $\text{Δ}g\left(r,\tau \right)=g\left(r,\tau \right)-g\left(r,{\tau }_{\max }=150s\right)$ for the examples shown in (b). (d) $\text{Δ}g\left(r,\tau \right)$ are fit to $\text{Δ}g\left(r,\tau \right)\propto \exp \left\{-r^2/4{\sigma }_{xy}^2\right\}$ to extract the LSF width in each lateral dimension. The timescale of the drift correction is shown in red. Error bars represent estimates of the standard error obtained through bootstrapping. The average resolution for this image is $⟨{\sigma }_{xy}⟩=$ 11.8 ± 1.5 nm. To see this figure in color, go online.