Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Parameter and conditon of eletroporation
| Organism | Cell lines | Tissue type | Cell numbers | Kit | Preset program | guide RNA |
|---|---|---|---|---|---|---|
| Mouse | B16-F10 | skin | 2 × 105 | SF | DJ-110 | crRNA/tracrRNA |
| Mouse | CT26 | colon | 2 × 105 | SE | DS-120 | sgRNA |
| Human | DU145 | prostate | 2 × 105 | SE | CA-137 | sgRNA |
| Human | PC-3 | prostate | 1 × 105 | SF | DS-137 | sgRNA |
| Human | MCF7 | breast | 4 × 105 | SE | EN-130 | sgRNA |
