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. 2022 Aug 18;8:106. doi: 10.1038/s41531-022-00369-w

Fig. 5. Overexpression of Esrrg in the midbrain causes an induction in mitochondrial genes and delays cell death in the PFF model of synucleinopathy.

Fig. 5

a sm-FISH for Th (red) and Esrrg (green) in mice with AAV:Gfp or AAV:Esrrg midbrain injections, quantified in b (n = 6 mice/group; two-tailed unpaired t-test *p < 0.05). c, d sm-FISH quantification for nuclear encoded mitochondrial gene Cox4i1 and mitochondrially encoded gene mt-cytb (n = 6/group two-tailed unpaired t-test or unpaired nonparametric Kolmogorov–Smirnov test ****p < 0.0001). e sm-FISH for Th (red) followed by immunofluorescence for phosphorylated α-synuclein (p-syn; green) at 1 month post-injection (P.I.). f Mean pixel density (occupancy of the cytoplasm) for p-syn per neuron at 1, 3 and 6 months P.I. (n = 4–6 mice/group; two-tailed unpaired t-test at each time-point **p < 0.005). g Percent of Th+ with presence of an inclusion for p-syn (n = 4–6 mice/group; two-tailed unpaired t-test at each time-point *p < 0.05, **p < 0.005). h, i Immunofluorescence for TH or DAT in the striatum of mice injected with AAV:Gfp or AAV:Esrrg and/or monomer or PFFs (n = 6 mice/group; mixed-effects analysis with Sidak’s post hoc analysis at each time-point *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001). j Neurons positive for TH immunoreactivity in mice injected with AAV:Gfp or AAV:Esrrg and/or monomer or PFFs (n = 6 mice/group; mixed-effects analysis with Sidak’s post hoc analysis at each time-point *p < 0.05, **p < 0.05). k, l Scatter plots to graph SNc TH neuron count and striatal TH intensity per animal at 3 and 6-months P.I. Numbers on bars are cell counts from each experiment. Scale bars correspond to 50 µm (a),100 µm (e, j), and 500 µm (h, i). Error bars represent ±SEM.