Fig. 1. Cryo-EM analysis of GlyR gating and THC modulation.

a Schematic representation of GlyR gating involving transitions from the Apo (resting) to glycine-bound, open and desensitized conformations. b TEVC recording of GlyR currents activated by 0.1 mM glycine, and with co-application of 3.2 μM Δ⁹-tetrahydrocannabinol (THC). Chemical structure of THC. c Cryo-EM 3D reconstructions of various GlyR conformations captured as a function of THC and Gly concentrations. A heuristic score is provided in parenthesis alongside each structure⁹⁵. A cut-off value of Σd > 0.55 is used for predicting a non-conductive conformation. GlyR-Apo and GlyR-5gly were previously reported¹⁹.