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. 2022 Aug 5;9:880358. doi: 10.3389/fmolb.2022.880358

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Copyright © 2022 Ahmad.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) GdmCl-induced denaturation curve monitored by CD₂₂₂ of α-phycoerythrin at pH 7.0 and 25°C. The protein concentration was 0.75 mg/ml and path length of the cell was 0.1 cm. The linear dependence of the pre-transition baseline is described by the relation, CD₂₂₂ = -152.6 + 2.90 [GdmCl]. The linear dependence of the post-transition baseline is described by the relation, CD₂₂₂ = -40.5 + 3.57 [GdmCl]. (B) Plot of ΔG _D versus [GdmCl]. Observed data (filled circles) were analyzed using three models of denaturation (see text). The best fit parameters are as follows: ΔG _D ⁰ = 8.58 (±0.20) kcal/mole and m = 3.94 (±0.09) kcal/mole. M for the linear model (curve 1); ΔG _D ⁰ = 15.03 (±0.46) kcal/mole, Δn = 48 (±1), and k = 0.7 for the binding model (curve 2); and ΔG _D ⁰ = 12.90 (±0.14) kcal/mole and Δα = 0.31 (±0.01) for the transfer-free energy model.