TABLE 3.
Respiratory rates of Synechocystis sp. strain PCC 6803 mutants lacking one or more NDH-2s
| Genotype of strain | Oxygen uptake (μmol of O2 mg of chl−1 h−1)a
|
|
|---|---|---|
| Wild type | PS I-less | |
| Parent | 50 ± 11 | 386 ± 49 |
| ΔndbA | 69 ± 15 | 470 ± 66 |
| ΔndbB | 77 ± 3 | 542 ± 74 |
| ΔndbC | 84 ± 14 | 377 ± 69 |
| ΔndbA ΔndbB | 81 ± 17 | 545 ± 110 |
| ΔndbA ΔndbC | 89 ± 10 | 524 ± 78 |
| ΔndbB ΔndbC | 93 ± 20 | 512 ± 142 |
| ΔndbA ΔndbB ΔndbC | 88 ± 17 | 509 ± 136 |
a
Values given are averages ± standard deviations from at least three determinations. Oxygen consumption of whole cells was measured in 10 mM HEPES–NaOH (pH 7.0). Strains in the wild-type background were grown photomixotrophically at 50 microeinsteins m−2 s−1, while those in the PS I-less background were grown photoheterotrophically at 5 microeinsteins m−2 s−1. chl, chlorophyll.