Table 1.
In vitro efficacy of ELQs against metacestodes and GL cells of two isolates of E. multilocularis.
| ELQ | Isolate Sval | Isolate H95 | ||
|---|---|---|---|---|
| Metacestodes | GL cells | Metacestodes | GL cells | |
| 100 | 0 (0.4) | 57.9 (5.7) | 1.4 (0.1) | 100.1 (8.3) |
| 121 | 20.9 (15.3) | 7.3 (3.8) | 15.2 (4.0) | 1.0 (0.3) |
| 127 | 2.5 (0.8) | 40.4 (2.7) | 11.7 (0.2) | 17.2 (9.5) |
| 136 | 7 (7.6) | 15.1 (4.7) | 21.4 (2.2) | 8.4 (11.7) |
| 271 | 56.9 (4.3) | 25.4 (7.2) | 33 (1.7) | 2.7 (1.6) |
| 300 | 7.6 (1.1) | 46.9 (9.6) | 6.6 (2.2) | 84.0 (7.0) |
| 316 | 4.6 (1.3) | 58 (5.3) | 21.4 (0.1) | 81.1 (9.5) |
| 400 | 27 (10.5) | 14.6 (8.6) | 21.4 (2.5) | 8.4 (4.8) |
| 433 | 1.8 (1.6) | 62.1 (6.3) | 4.3 (4.2) | 96.4 (17.7) |
| 434 | 0.6 (1.1) | 81.3 (5.8) | 0.4 (0.4) | 33.3 (6.4) |
| 435 | 0 (0.9) | 80.1 (13.1) | 2.6 (1.6) | 15.6 (14.0) |
| 436 | 3.1 (2.6) | 16.4 (1.8) | 3.4 (1.2) | 14.0 (9.7) |
| 437 | 33.1 (7.2) | 19.1 (5.7) | 37.6 (0.5) | 19.7 (13.3) |
ELQ numbers are given in the first column. The two parasite isolates Sval and H95 were tested. Efficacy against metacestodes was assessed by PGI assay (relative to the positive control Tx-100), and against GL cells by viability assessment via ATP measurements (relative to the DMSO control) in μM. Compounds were considered to be active, if they reached a threshold of >20% against metacestodes, and <30% against GL cells. This overview screen was performed once in triplicates, and respective mean relative values are given with standard deviations in parentheses.