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. 2022 Aug 19;44(3):561–572. doi: 10.1038/s41401-022-00968-6

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© The Author(s), under exclusive licence to Shanghai Institute of Materia Medica, Chinese Academy of Sciences and Chinese Pharmacological Society 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

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Fig. 1 — Rats were intraperitoneally injected with various doses of ZNE once a day for 4 weeks. a Body weight changes of rats were recorded. b, c The changes in HW/BW and HW/TL were measured and counted. d Systolic and diastolic functions of the heart in rats were observed by echocardiography. e, f EF and FS of heart in rats were calculated. g, i LVPW thickness and IVS thickness of heart in rats were measured during diastole. h, j LVPW thickness and IVS thickness were measured during systole. k H&E staining (×100) of cross-sections of ventricles of rats was observed under a microscope, and the average area of a single cardiomyocyte was analyzed statistically. The red area represented cytoplasm of cardiomyocytes while the blue area represented nuclei of cardiomyocytes. Scale bar: 100 μm. n = 5 rats. Data are expressed as mean ± SEM, *P < 0.05, **P < 0.01, ***P < 0.001 vs Control.