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. 2022 Aug 19;44(3):561–572. doi: 10.1038/s41401-022-00968-6

Fig. 8. NC regulates autophagy by targeting ATG4B, affecting cardiomyocyte hypertrophy.

Fig. 8

a After NMCMs were transfected with ATG4B for 48 h, the overexpression efficiency of ATG4B was verified by Western blot. n = 5 cells. NMCMs were transfected with ATG4B for 24 h and treated with NC for 48 h. b The cell viability was determined using the CCK8 kit. n = 7 cells. c The cytoskeleton of NMCMs was stained with F-actin, and the area of a single cardiomyocyte was statistically analyzed. Scale bar: 50 μm. n = 3 cells. d, e The expression levels of autophagy-related proteins LC3 I, LC3 II, LC3 II/LC3 I, and p62 in NMCMs were quantified by Western blot analysis. n = 5 cells. Data are expressed as mean ± SEM, **P < 0.01, ***P < 0.001 vs Control. #P < 0.05, ##P < 0.01, ###P < 0.001 vs NC (10 μM).