. 1999 Jul;181(13):4004–4011. doi: 10.1128/jb.181.13.4004-4011.1999

TABLE 2.

Effects of replacement of the signal anchor sequence of PulC

Plasmid^a	Protein	Sequence^b	Complementation of ΔpulC
Plasmid^a	Protein	Sequence^b	Noninduced	Induced
pCHAP1305	PulC	LAQLTWEFFKIIL	80	100
pCHAP1232	MalE-′PulC	IEGRISEFFKIIL	0	0
pCHAP1292	MalE^SP-′PulC	A^AKIDLEFF*KIIL	30	80
pCHAP1315	MalE^SA-′PulC	DAKIDLEFFKIIL	50	80
pCHAP1316	PhoE^SP-′PulC	ASVQA^AEFF*KIIL	80	100
pCHAP1298	PulA^SPCD-′PulC	G^CDNSAEFF*KIIL	30	100
pCHAP1299	PulA^SPCS-′PulC	G^CSNSAEFF*KIIL	0	0

All are derivatives of pSU18 (2), with the gene fusion under lacZp control (induced with 1 mM IPTG).

Sequences indicated are at the junction between the sections of the protein near the beginning of the periplasmic domain of PulC. Residues in italics are derived from MalE, PhoE, or PulA and linker-encoded residues; other amino acids are from PulC. The last residue of the PulC sequence indicated is position 50. The sequence of unmutated PulC upstream of this position is LAQLTWKIIL. ∗, signal peptidase cleavage site.