Skip to main content
. 2022 Aug 5;13:941608. doi: 10.3389/fimmu.2022.941608

Figure 1.

Figure 1

Increased TIM-4 expression in macrophages from patients with CRSwNP. (A) The mRNA expression levels of TIM-4 in different human sinonasal mucosae, as detected by quantitative RT–PCR. (B-E) Protein expression levels of TIM-4 in the human sinonasal mucosa. (B) The protein levels of TIM-4 in the human sinonasal mucosa from the control, CRSsNP, E-CRSwNP, and NE-CRSwNP groups, as detected by Western blot analysis. GAPDH was used as a control. (C) Quantitative summary of the relative protein expression of TIM-4. (D) The TIM-4+ cells and CD68+ cells in the human sinonasal mucosa from the control, CRSsNP, E-CRSwNP, and NE-CRSwNP groups were examined by IHC. (E) Quantitative summary of the number of TIM-4+ cells in the human sinonasal mucosa. (F) Quantitative summary of the number of CD68+ cells in the human sinonasal mucosa. (G, H) Colocalization of TIM-4+ and CD68+ in the human sinonasal mucosa. (G) Double immunofluorescence staining of TIM-4 (green) and CD68+ macrophages (red) in the lamina propria of human sinonasal mucosa from the control, CRSsNP, E-CRSwNP, and NE-CRSwNP groups. (H) Quantitative summary of the number of TIM-4+/CD68+ cells in the human sinonasal mucosa. RT–PCR, IHC (control group: n=8; CRSsNP group: n=11; E-CRSwNP group: n=11; NE-CRSwNP group: n=9); Western blot, and immunofluorescence (control group: n=6; CRSsNP group: n=6; E-CRSwNP group: n=6; NE-CRSwNP group: n=6). All of the above representative pictures are shown at a magnification 400×, and the insets show a higher magnification of the selected area. Bars show the mean ± SD. *P<0.05, **P<0.01,***P<0.001.