FIG. 3.

Complementation of the xcpP mutation in PAO1Δ with plasmids carrying recombinant genes encoding variants of XcpP. (A) Immunodetection of elastase in whole-cell extracts (C) or culture supernatants (SN) of PAO1ΔP containing pMMB190 or a plasmid encoding XcpP (pSB10), XcpPΔM (pSB74), or Tc-′XcpP (pSB62). Elastase is indicated by the arrow. (B) Immunodetection, using anti-XcpP antiserum, of variants of XcpP produced in PAO1ΔP containing pSB24 (ss-′XcpPp; precursor (°) and mature (*) forms), pSB62 (Tc′-XcpP), pSB10 (°XcpP and *XcpP*), pSB74 (XcpPΔM), pSB86 (XcpPΔ19), or pMMB190. In both cases, proteins were separated by electrophoresis in an 11% acrylamide gel.