FIG. 5.

Competitive inhibition assay. Samples of PAO1 cells containing pMMB190 (A), pSB24 encoding ss-′XcpPp (B), or pSB51 encoding ss-′XcpZp (C) were taken at various times (indicated in hours) after addition of IPTG. Cells and supernatants were separated, and their protein contents were analyzed by electrophoresis in an 11% acrylamide gel and transfer onto nitrocellulose sheets. The blots were probed with an antielastase antiserum. The elastase band is indicated; the highest-molecular-weight band is a product of cross-reaction.