FIGURE 6.

Injury efficacy of cell-free supernatant (CFS) on the hyphal cytomembrane of Botrytis cinerea in vitro. (A) Detecting the integrity of membrane of hyphal cells at 24 h after the application of CFS was stained by propidium iodide (PI). The fluorescence signal was detected using a laser scanning confocal microscope at 405 nm excitation and 633 nm emission. (B) The relative intensity of fluorescence of each treatment was measured by ImageJ. (C) Malondialdehyde (MDA) content which refers to the degree of membrane lipid peroxidation of hyphal cells, was recorded after treatment with CFS for 24 h. (D) Total protein content in hyphae culture medium after a different amount of CFS treatment. (E) Nucleic acid content as measured by A₂₆₀ in hyphae culture medium after different amounts of CFS treatment. Vertical bars represent standard deviations of the means. Treatments followed by different letters are statistically different by the Duncan’s multiple range (p < 0.05).