FIG. 7.

Fluorographic detection of M. catarrhalis UspA1 and UspA2 proteins expressed in vitro. The M. catarrhalis O35E uspA1 gene (lane 1), the O35E uspA2 gene (lane 2), the TTA24 uspA1 gene (lane 3), and the TTA24 uspA2 (lane 4) genes were each amplified from the chromosome of their respective M. catarrhalis strains and used in an in vitro coupled transcription-translation system for linear DNA templates to establish the size (as determined by SDS-PAGE) of the protein product encoded by each gene. Radiolabeled (¹⁴C-labeled) standards (not shown) were used to determine the position of the molecular mass markers (in kilodaltons) present on the left side of the figure. Degradation products are visible beneath each primary protein product.