Figure 1.

Quantification of phalloidin-defined area using ImageJ

(A) Example of images acquired at the confocal microscope. Left image represents a spread cell with complex cortical phalloidin staining (red). The right image shows cells that do not spread and have defects in cortical cytoskeleton structuring. Red is polymerized actin stained with phalloidin; Blue is nucleus stained with Dapi. Scale bar 10 μm.

(B) Screenshot of the final step of the ImageJ-based analysis used to quantify the area defined by the red channel staining and the intensity of the staining.