REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Alexa Fluor 594 phalloidin	Invitrogen	A12381
Bacterial and virus strains
Ecc15 (2141)	Dr. Edan Foley, University of Alberta	Basset et al., 2000
Chemicals, peptides, and recombinant proteins
SFM4 Insect Cell Culture Medium	Hyclone	SH30913.02
penicillin/ streptomycin sulfate 10× solution	Thermo Fisher Scientific	15140122
4,6-diamidine-2-phenylindole (DAPI)	Invitrogen	D1306
Pro-Gold Antifade Reagent	Invitrogen	P36930
Concanavalin A	MP Biomedicals	CAT 195283
Trypan Blue stain (0.4%)	Gibco	REF 15250-061
Calcium–magnesium-free Phosphate Buffer Saline (PBS)	Corning	20-031-CV
Normal goat serum	Sigma-Aldrich	NS02L
Triton X-100	Sigma-Aldrich	CAT 9002-93-1
16% Formaldehyde	Thermo Scientific	28906
cOmpleteTM Protease Inhibitor Cocktail	Roche	04693116001
Cellfectin II reagent	Invitrogen	10362-100
Tissue and Blood DNA extraction kit	QIAGEN	69504
Experimental models: Cell lines
Drosophila melanogaster S2R+	DGRC S2R+ (https://dgrc.bio.indiana.edu//stock/150; RRID:CVCL_Z831)	Stock Number: 150
Experimental models: Organisms/strains
Drosophila melanogaster	Bloomington Drosophila Stock Center (https://bdsc.indiana.edu/Home/Search)	#30142
Oligonucleotides
Gene-specific primer with anchor for PCR#1	5′-GGGCGGGT (followed by gene specific sequence)	5′-GGGCGGGT (followed by gene specific sequence)
T7 primer	5′ TAATACGACTCACTATA GGGAGACCACGGGCGGGT	5′ TAATACGACTCACTATA GGGAGACCACGGGCGGGT
Software and algorithms
FIJI/ImageJ software	Schindelin et al., 2012	https://imagej.nih.gov/ij/
Icy Bioimage	BioImage Analysis Lab at Institut Pasteur	http://icy.bioimageanalysis.org
Zeiss Zen lite Black & lite Blue	ZEISS	https://www.zeiss.com/microscopy/int/products/microscope-software/zen-lite.html?vaURL=www.zeiss.com/zen-lite
Other
12 mm diameter round glass coverslips	Ted Pella	#2603
incubator for Drosophila culture	DigiTherm Tritech Research	DT2-MP-47
Bench-top centrifuge	Eppendorf	5427 R
Bench-top centrifuge	Eppendorf	5810R
Thermocycler	Applied Biosystems	SimplyAMP
24-well tissue culture-treated plates	Falcon	38021
6-well tissue culture-treated plates	Falcon	353046
Surgical forceps	Fine Science Tools, Dumont	#55
Pyrex dissecting dishes	Corning	7220-85
8-chamber cover glass	Nunc	155411
Confocal microscope	ZEISS	LSM 880 with Airy scan
Stereomicroscope	ZEISS	STEMI
glass beads	Sigma-Aldrich	G8772