| Step | Temperature | Time |
|---|---|---|
| 1 | 95°C | 3 min |
| 2 | 95°C | 30 s |
| 3 | 50°C | 45 s |
| 4 | 72°C | 1 min |
| 5 | Go to step 2, 39× more times | |
| 6 | 72°C | 10 min |
| 7 | Pause at 4°C |
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