Reagents	Volume
PCR #1 product	10 μL
T7 linker primer (10 μΜ)	2 μL
dNTP (25 mM each)	0.5 μL
10× Taq PCR Buffer (NEB)	5 μL
Taq	0.5 μL
Molecular grade sterile water	32 μL
Total	50 μL