Fig. 2.
NO metabolism and antioxidant defense upon STOX1 overexpression and hypoxia. (A) Quantification of inducible NOS (iNOS; NOS2) fluorescence intensity per cell (images in Fig. S2A). Assessment of (B) Nitric oxide (NO) using the DAF-2 DA probe, (C) the ROS anion superoxide (O2−) using the DHE probe, and (D) the RNS peroxynitrite (ONOO−) using the DHR123 probe, expressed as percentage of the untreated control BD3 cell line. (E) Total SOD activity (measured as inhibition of the activity of SOD, percentage). (F) Catalase activity expressed in nmol/min/ml. Immunofluorescence, n = 60 cells from three independent experiments, mean ± SD. Dosages (NO, O2−, peroxynitrite), n = 3–6. Enzyme activities (SOD, catalase), n = 6 for untreated BD3/AA6/B10 in NO and O2− assays, n = 3 independent experiments for all other experiments, mean ± SD, *p ≤ 0.05 **p ≤ 0.01 ***p ≤ 0.001 based on one-way ANOVA versus the untreated control (red stars) or the corresponding untreated condition (blue stars). (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)