FIG. 2.

FPLC gel filtration chromatography on Superose 12 HR column of the cell-free fraction of C. cellulolyticum grown on xylan. The fractions were analyzed for protein content (■) and xylanase activity (●) under the following conditions: protein load, 0.38 mg; flow rate, 0.5 ml/min; and buffer, 100 mM Tris-HCl (pH 7.0). Arrows indicate the retention times of some standards: a, blue dextran (indicating void volume); b, thyroglobulin (669 kDa); c, apoferritin (443 kDa); d, β-amylase (200 kDa); e, albumin (66 kDa); f, carbonic anhydrase (29 kDa); g, total volume.