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A-C were the images of c-Fos mapping from a representative animal in SD group. (A) Sagittal plane; (B) Coronal plane; (C) Horizontal plane. Scale bar, 2000 μm. (D) The top 27 brain regions with more than 2000 c-Fos + expressing cells in the chronic sleep deprivation group. N = 3，Student's t-test. Error bars indicate s.e.m.
