The number of c-Fos + expressing cells in the anterior cingulate area (ACA).
(A, C, D) ACA was located at the isocortex. Green marks the ACA according to Allen Mouse Brain Common Coordinate Framework V3. (B, E, F) The number of c-Fos + cells in the ACA of the sleep deprivation group was more than that of the control group. Red marks the c-Fos + cells. (G, H) The enlarged view from (E, F). Scale bar: (A–F) 2000 μm, (G, H) 6000 μm. N = 3,Student's t-test. Error bars indicate s.e.m. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)