Skip to main content
. 2022 Jul 23;33(8):1494–1504. doi: 10.1021/acs.bioconjchem.2c00220

Figure 2.

Figure 2

FcRn binding and transport properties of the Quad molecules. (A, B) FcRn-ELISA binding assays were obtained for NIP-IgG1-WT, Q187, Q190, and Q191 at acidic pH (pH 5.5) and neutral pH (pH 7.4). (C) Schematic overview of the HERA protocol. Quads and anti-NIP-IgG1 were added to starved HMEC1-hFcRn cells (1–2) and incubated for 3 h to allow for uptake (3), followed by lysis. Samples were removed, followed by a new 3 h incubation period with fresh medium to allow recycling and release into the medium, or retention inside the cells measured after lysis of the cells (4). Proteins present in the lysates and recycling medium were quantified by two-way anti-Fc ELISA (5). The figure was created with Biorender.com. (D–F) ELISA quantification of the amounts taken up, recycled, or accumulated. Data represents three independent experiments; mean ± SD, unpaired Student’s t-test: *p > 0.05, **p > 0.01, ***p > 0.001, **** p > 0.0001.