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. 2022 Jul 23;33(8):1494–1504. doi: 10.1021/acs.bioconjchem.2c00220

Figure 3.

Figure 3

Apparent affinity and blocking characterization of Quad molecules to α-cbtx. (A) Blocking of the α-cbtx/AChR interaction with Quad molecules in an ELISA-based assay. Each data point represents the mean of two independent experiments ± SD. (B) Comparison between apparent KD and IC50 blocking potency. * Denotes Quad formats that had increased interaction with the FIDA capillary. (C) Representative isothermal titration calorimetry thermograms and curve fits for titrations of VHH, Q187, Q189, and Q207/208 into α-cbtx. Binding affinity (KD) and stoichiometry (N) are the average of two independent titrations.