Table 1. Blocking Potency, Size-Exclusion Chromatography, and Production Analysis of the Different Quad Formats.
| blocking |
SEC
analysis |
production | |||
|---|---|---|---|---|---|
| molecule (valency) | IC50 (nM) | VHH/Quad | main peak (%) | elution (mL) | yield (mg/L) |
| VHH (1) | 0.80 | 1 | 100.0 | 17.56 | 83 |
| Q187 (4) | 0.18 | 4.7 | 97.0 | 13.15 | 83 |
| Q188 (8) | 0.11 | 7.7 | 97.0 | 11.48 | 75 |
| Q189 (8) | 0.10 | 8.7 | 95.0 | 11.90 | 108 |
| Q190 (4) | 0.21 | 3.9 | 88.5 | 10.09 | 125 |
| Q191 (8) | 0.13 | 6.3 | 98.0 | 9.56 | 65 |
| Q193 (4) | 0.19 | 4.5 | 81.0 | 11.75 | 67 |
| Q194 (8) | 0.11 | 7.4 | 86.0 | 10.65 | 37 |
| Q207/208 (16) | 0.05 | 15.4 | 98.6 | 9.17 | 100 |