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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Nat Microbiol. 2021 Oct 25;6(11):1410–1423. doi: 10.1038/s41564-021-00973-1

Extended Data Fig. 8∣. Desensitization of M. tuberculosis H445Y RNAP to ppGpp in vitro.

Extended Data Fig. 8∣

a, In vitro transcription reactions of M. tuberculosis wild type and RifR rpoB H445Y RNAP with or without ppGpp (10 mM). Stalled RNAP elongation complexes (20-mer, denoted with the red arrow) were chased to the end of the template (runoff, denoted with the blue arrow), and the labeled nascent RNA then separated using PAGE. For in vitro transcription experiments, n=3 independent replicates. b, Quantification plot of signal from the gel scans as a function of distance from the initial site of stalling (right-end on the x-axis). Arrows (labeled 1 through 5) correspond to major pause sites.