TABLE 1.
Plasmids
| Plasmid | bpa | Vectorb | Relevant genotypec | Source or reference |
|---|---|---|---|---|
| pACYC177 | Apr Kmr | 6 | ||
| pBGS18 | Kmr | 33 | ||
| pGB2 | Spr Smr | 7 | ||
| pLITMUS28 | Apr | New England Biolabs | ||
| pLITMUS29 | Apr | New England Biolabs | ||
| pRS552 | Apr Kmr | ′lacZ | 32 | |
| pAY50 | 1–8072 | pBGS18/Kmr | 18 | |
| pAY60 | 4585–8072 | pBGS18/Kmr | 28 | |
| pAY62 | 4585–8072 | pBGS18/Kmr | This study | |
| pAY721 | 4585–6809 | pBGS18/Kmr | uoi+ int+ attP+ | This study |
| pAY952 | 4585–6809 | pGB2/Spr Smr | uoi+ int+ attP+ | This study |
| pAY979 | 4585–6809 | pBGS18/Kmr | intVA42 | 28 |
| pAY754 | 4585–6809 | pBGS18/Kmr | intVA1 | 28 |
| pAY990 | 4585–6809 | pBGS18/Kmr | intVA1 intVA42 | 28 |
| pAY722 | 4808–6809 | pBGS18/Kmr | uoi+ int+ attP+ | This study |
| pAY759 | 4979–6809 | pBGS18/Kmr | uoi+ int+ attP+ | This study |
| pAY743 | 4585–5403 | pLITMUS29/Apr | uoi-14(Am) Pint-14 | This study |
| pAY951 | 4585–6809 | pBGS18/Kmr | uoi-14(Am) Pint-14 | This study |
| pAY995 | 4585–6809 | pBGS18/Kmr | uoi-14(Am) Pint-14 | This study |
| pAY950 | 4585–6809 | pBGS18/Kmr | uoi+ int+ attP+ | This study |
| pAY735 | 4585–6809 | pBGS18/Kmr | uoi+ int-nd attP+ | This study |
| pAY980 | 4585–6809 | pBGS18/Kmr | uoi+ Δint-5403/5851 attP+ | This study |
| pAY725 | 4585–6809 | pBGS18/Kmr | int-1(Am) | This study |
| pAY982 | 4585–6809 | pBGS18/Kmr | int-1(Am) | This study |
| pAY703 | pBGS18/Kmr | mglBA+ | 18 | |
| pAY971 | 5073–6809 | pBGS18/Kmr | mglBA+int+ attP+ | This study |
| pAY972 | 5208–6809 | pBGS18/Kmr | mglBA+int+ attP+ | This study |
| pAY987 | 5238–6809 | pBGS18/Kmr | mglBA+ Δint-5238 | This study |
| pAY992 | 5298–6809 | pBGS18/Kmr | mglBA+ Δint-5298 | This study |
| pAY993 | 5337–6809 | pBGS18/Kmr | mglBA+ Δint-5337 | This study |
| pAY733 | 4585–6504 | pBGS18/Kmr | Δint-6504 | This study |
| pAY734 | 4585–6711 | pBGS18/Kmr | Δint-6711 | This study |
| pAY146 | 4585–6716 | pBGS18/Kmr | Δint-6716 | This study |
| pAY997 | 1–8072 | pACYC177/Kmr | uoi+ int-NotI′ attP+ | This study |
| pAY738 | 4585–5210 | pRS552/Apr Kmr | uoi+ int-lacZ (fusion to GTG-5085) | This study |
| pAY739 | 4585–5087 | pRS552/Apr Kmr | Δuoi int-lacZ (fusion to GTG-5208) | This study |
a
Coordinates of Mx8 DNA are those of GenBank accession no. U64984.
b
Antibiotic resistance determinants retained from the vector are indicated.
c
The uoi-14 mutation inactivates the int promoter (Pint); int-nd is an uncharacterized PCR-induced mutation within bp 4585 to 6089 that inactivates the int gene. The int-NotI′ mutation is a 4-bp insertion within int made by filling in the NotI site and ligation.