TABLE 1.
Plasmids
| Plasmid | bpa | Vectorb | Relevant genotype | Source or reference |
|---|---|---|---|---|
| pBGS18 | Kmr | 31 | ||
| pAY1099 | Spr Smr | 18 | ||
| pLITMUS28 | Apr | New England Biolabs | ||
| pLITMUS29 | Apr | New England Biolabs | ||
| pAY721 | 4585–6809 | pBGS18/Kmr | uoi+ int+ attP+ | This study |
| pAY952 | 4585–6809 | pGB2/Spr Smr | uoi+ int+ attP+ | This study |
| pAY980 | 4585–6809 | pBGS18/Kmr | uoi+ Δint-5403/5851 attP+ | 19 |
| pAY994 | 5851–6809 | pBGS18/Kmr | attP+ | This study |
| pAY999 | 4585–6469 | pBGS18/Kmr | uoi+ intX+ attR+ | This study |
| pAY1311 | 5851–6469 | pBGS18/Kmr | attR+ | This study |
| pAY1299 | 5851–6809 | pLITMUS28/Apr | attP5 | This study |
| pAY1300 | 4585–6809 | pBGS18/Kmr | attP5 | This study |
| pAY971 | 5073–6809 | pAY703/Kmr | int+-5085 | This study |
| pAY972 | 5208–6809 | pAY703/Kmr | int+-5208 | This study |
| pAY973 | 4979–6809 | pAY703/Kmr | uoi+ int+ | This study |
| pAY1301 | 5073–6809 | pAY1099/Spr Smr | int+-5085 | This study |
| pAY1302 | 5208–6809 | pAY1099/Spr Smr | int+-5208 | This study |
| pAY1303 | 4979–6809 | pAY1099/Spr Smr | uoi+ int+ | This study |
| pAY1304 | 5073–6809 | pAY1099/Spr Smr | int+-5085 attP5 | This study |
| pAY1305 | 5208–6809 | pAY1099/Spr Smr | int+-5208 attP5 | This study |
| pAY1306 | 4979–6809 | pAY1099/Spr Smr | uoi+ int attP5 | This study |
| pAY1307 | 5851–6469 | pLITMUS28/Apr | attR5 | This study |
| pAY1308 | 5073–6469 | pAY1099/Spr Smr | intX+-5085 attR5 | This study |
| pAY1309 | 5208–6469 | pAY1099/Spr Smr | intX+-5208 attR5 | This study |
| pAY1310 | 4979–6469 | pAY1099/Spr Smr | uoi+ intX+ attR5 | This study |
| pAY1048 | 5851–6469 | pBGS18/Kmr | ΔattP-1048 | This study |
| pAY1044 | 6398–6469 | pBGS18/Kmr | ΔattP-1044 | This study |
| pAY1046 | 6398–6809 | pBGS18/Kmr | ΔattP-1046 | This study |
| pAY1042 | 6447–6809 | pBGS18/Kmr | ΔattP-1042 | This study |
| pAY1041 | 6447–6469 | pBGS18/Kmr | ΔattP-1041 | This study |
| pAY1039 | pBGS18/Kmr | attB1+ attB2+ | This study | |
| pAY1037 | pLITMUS28/Apr | attL1+ | This study | |
| pAY1038 | pBGS18/Kmr | attL1+ | This study |
a
Coordinates of Mx8 DNA are those of GenBank accession no. U64984.
b
Antibiotic resistance determinants retained from the vector are indicated.