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The IntX integrase can promote integrative (attP × attB) recombinationa
| Plasmid(s) [relevant genotype(s)] | EOE (103 μg−1) |
|---|---|
| pAY980 (Δint-5403/5851 attP+) | <0.005 |
| pAY994 (attP+) | <0.005 |
| pAY999 (intX+ attR+) | 0.002 |
| pAY999 (intX+ attR+) + pAY980 (Δint-5403/5851 attP+) | 0.07 |
| pAY999 (intX+ attR+) + pAY994 (attP+) | 0.17 |
The efficiency of electroporation (EOE) of each plasmid alone or in coelectroporations was measured as the numbers of Kmr recombinants of host DZ1 as described elsewhere (19).
