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. 2022 Aug 19;12:132. doi: 10.1186/s13578-022-00866-8

Table 1.

Composition of the culture medium for urological cancer organoids

Types of organoids Culture medium
Generic ingredients DMEM/F12
EGF
Y-27632(ROCK inhibitor)
A83-01
Prostate cancer Prostaglandin E2
DHT
B27
N-acetylcysteine
Noggin
R-spondin1
FGF10
FGF2
Nicotinamide
SB202190
Bladder cancer FGF7
FGF10
N-acetylcysteine
Nicotinamide
N-21
Heregulin
CHIR99021
Renal cancer Heparin
Amphotericin B
bFGF
B27
N-acetylcysteine

EGF epidermal growth factor, ROCK Rho-related protein kinase, DHT dihydrotestosterone, FGF fibroblast growth factor