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. 2022 Aug 19;41:252. doi: 10.1186/s13046-022-02467-2

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — SLERCC directly binds to UPF1. A FISH assay to assess the intracellular localization of SLERCC in ACHN and 786-O cells. B qRT-PCR analysis of SLERCC in nuclear and cytoplasmic fractions of ACHN and 786-O cells. C RNA pull-down assay for SLERCC in ACHN cells. D Mass spectrometric (MS) analysis of the proteins from the RNA pull-down assay. E Western blotting after RNA pull-down with recombinant UPF1. F Agarose gel electrophoresis for the RNA pull-down assay shows that UPF1 is a direct target of SLERCC. G RIP assay using anti-UPF1 to assess the enrichment of SLERCC by UPF1. IgG is the negative control, while U1 is a nonspecific control. H, I Serial deletion RNA pull-down assay for SLERCC in ACHN cells. J, K RIP assay after deletion of 181–360 nt from SLERCC in ACHN and 786-O cells. L Prediction of the stem-loop structures at UPF1-binding sites in SLERCC. M PyMOL software displaying the interaction between SLERCC and UPF1 in their 3D protein structures. N FISH to assess the colocalization of SLERCC and UPF1 in ACHN and 786-O cells. (***p < 0.001). Abbreviations: TCGA, The Cancer Genome Atlas; FISH, Fluorescence in situ hybridization; RIP, RNA immunoprecipitation