Fig. 3.

Human MDMs with higher lncMMPA increase aerobic glycolysis and decrease apoptosis of HCC cells. A-G Co-culture of Hep3B cells with pri-TAMs, MDMs-control or MDMs-lncMMPA was done in Transwell systems for 6 days. Then the Hep3B cells were harvested for the specified experiments. A Glucose consumption. B Lactate production. C ECAR. D Cell proliferation. E Expression of GLUT1, HK2 and LDHA. F The representative plots of flow cytometry analysis for Annexin V-APC and propidium iodide staining in Hep3B cells treated with Adriamycin (2 μg/ml) for 48 h. G The quantification of flow cytometry analysis in F. H-L Co-culture of Hep3B cells with TAMs-shControl or TAMs-shlncMMPA was done in Transwell systems for 6 days and then the Hep3B cells were harvested for the following experiments. H Glucose consumption. I Lactate production. J ECAR. K Cell proliferation. L Expression of GLUT1, HK2 and LDHA