Fig. 1.

2D versus 3D culture system for BM-MSCs. a Schematic diagram of BM-MSCs grown in a 2D and 3D culture conditions. Cells were either grown in 2D culture flask or 3D bioreactor on microcarriers for expansion. Cells were transferred to collection medium for 3 days and then collected and processed for EV isolation using low and high-speed centrifugation steps. b Morphology of BM-MSCs after 3 days in collection medium and c bioreactor, microcarriers and cell attachment and expansion around microcarriers. d The statistical analysis illustrated significant expansion in cell number in 3D versus 2D culture conditions (P < 0.05). Scale bar 200 px