FIG. 1.

Proton uptake by washed inverted vesicles from M. mazei Gö1. The experiments were performed as described in Materials and Methods. The amount of translocated protons was calculated from the difference between maximal alkalinization and the final baseline after reacidification. The reaction was started by pulses of 2-OH-phenazine or dihydro-2-OH-phenazine as indicated. SF 6847 was added as an ethanolic solution to a final concentration of 15 nmol/mg of protein. (A) H₂-dependent reduction of 2-OH-phenazine under an atmosphere of molecular hydrogen; (B) dihydro-2-OH-phenazine-dependent reduction of CoM-S-S-CoB under an atmosphere of molecular nitrogen.