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. 2022 Aug 19;12:14173. doi: 10.1038/s41598-022-18448-6

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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NEU and PPCA Gene Expression During HL60 Cell Differentiation. (A) Total RNA isolated from nondifferentiated HL60 cells was processed for qRT-PCR for mRNA levels for NEU1-4 and PPCA, and normalized to the 18S rRNA control. (B–F) Total RNA isolated from HL60 cells cultured for increasing times in the presence of DMF or medium alone was processed for qRT-PCR to quantify mRNA levels for NEU1 (B), PPCA (C), NEU2 (D), NEU3 (E), and NEU4 (F), and each normalized to the 18S rRNA control. The n for each data point is indicated below each vertical bar (A) or time point (B–F). Each vertical bar (A) or symbol (B–F) represents mean ± SE normalized mRNA levels. The data generated in each panel represents experiments performed on ≥ 2 independent occasions. *, increased normalized NEU2/4 mRNA expression compared to nondifferentiated cells at p < 0.05.