FIG. 3.
Western blot analysis of AOX. (A) Western blot analysis of AOX from fresh cells of CAI4 (lane 1), WH304 (lane 2), WH305 (lane 3), and WH306 (lane 4). (B) Western blot analysis of AOX from antimycin A-treated cells of CAI4 (lane 1) and WH304 (lane 2). (C) Western blot analysis of AOX from WH117 (lane 1) and WH119 (lane 2). Mitochondrial proteins (30 μg) were separated by SDS-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with a monoclonal antibody to AOX. Numbers on the left side of the immunoblots are molecular masses of the standards. The arrow on the right side of each immunoblot indicates AOX.