Figure E1.

Validation of mouse model of cardiac hypertrophy using transverse aortic constriction (TAC) according to gross pathology and fibrosis. The aortic arch of C57BL/6 mice was constricted between the innominate artery and left common carotid artery for 6 weeks. Sham mice underwent all surgical procedures except constriction of the aorta. A, Echocardiographic image showing aortic banding in a mouse; (B) validation of TAC-induced cardiac hypertrophy; and (C) Masson's trichrome-stained heart sections showing fibrosis elicited by TAC.