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Effect of scoC overexpression on opp (A) or app (B) transcription. Strain JH14279 (A) or JH14270 (B) carrying replicative plasmid pBS19 (○; vector only), pJM2491 (□); scoC), or pJM2493 (⧫; scoC promoter) was grown in Schaeffer’s sporulation medium containing chloramphenicol at 5 μg/ml. β-Galactosidase activity was measured at the indicated times.
