Table 2.
Sterilization conditions for frozen mice based on EN ISO 17665-1.
| Conditions | Temperature | Duration | Pressure | |
|---|---|---|---|---|
| Advanced sterilization | 1.0 Kelvin | |||
| Fractionated prevacuum stages | Stage 1 | Stage 2 | Stage 3 | Stage 4 |
| Number of repetitions | 1 | 1 | 4 | 4 |
| Pressure steam injection | 600 mbar /8.7 psi | 1000 mbar /14.5 psi | 1900 mbar /27.6 psi | 2500 mbar /36.3 psi |
| Pressure steam flush II | 90 mbar /1.3 psi | 90 mbar /1.3 psi | 1300 mbar /18.9 psi | 2000 mbar /29 psi |
| Flow steam flush II | 100% | 100% | 100% | 100% |
| Pressure evacuation | 75 mbar /1.1 psi | 200 mbar /2.9 psi | 1300 mbar /18.9 psi | 2000 mbar /29 psi |
| Pressure steam flush I | 90 mbar /1.3 psi | 215 mbar /3.1 psi | 1300 mbar /18.9 psi | 2000 mbar /29 psi |
| Time steam flush I | 60 s | 60 s | — | — |
| Flow steam flush I | 10% | 10% | 100% | 100% |
| Sterilization | 127 °C | 20 min | 2500 mbar / 36.3 psi | |
| Drying /Airflow | . | 3 min | 800 mbar / 11.6 psi | |
| Ventilation pressure | — | — | 1100 mbar / 16 psi | |
I: Evacuation and flow pressure used for lower pressure levels
II: Evacuation and flow pressure used for higher pressure levels
Advanced sterilization: This value is added to the set the sterilization temperature
Flow steam flush I: Opening percentage of the vacuum valve
Flow steam flush II: Opening percentage of the vacuum valve
Number of repetitions: This value indicates how often the process element (stage) is repeated with an identical procedure
Pressure steam injection: Set pressure for steam injection
Pressure evacuation: Set pressure for evacuation
Pressure steam flush II: Set pressure for steam flush II
Pressure steam flush I: Set pressure for steam flush I
Time steam flush I: Holding time at steam flush pressure I