Table 8. Biochemical effect of essential oil of C. citrinus on T. castaneum.
| BIOCHEMICAL STUDIES | Concentrations–(μL/L) | ANOVA | |||
|---|---|---|---|---|---|
| Control | 5 | 10 | 20 | ||
| Total protein (μg protein/μl of homogenate | 7.43±0.07 | 6.19±0.03 | 5.72±0.15 | 5.32±0.21* | Kruskal-Wallis Test p≤0.022 |
| Acetylcholinesterase (μM of AcT Hydrolysed/min/mg of protein) | 3.30±0.04 | 3.68±0.05 | 3.74±0.04 | 2.93±0.06* | Kruskal-Wallis Test (p ≤ 0.039) |
| α-Carboxylesterase (μM of α-Naphthol released/min/mg of protein) | 67.06±1.83 | 85.46±1.13 | 99.38±2.25 | 110.59±2.69* | Kruskal-Wallis Test (p ≤ 0.013) |
| β-carboxylesterase (μM of β-Naphthol released/min/mg of protein) | 0.056±0.0002 | 0.137±0.0009 | 0.137±0.0007 | 0.133±0.0015 | Kruskal-Wallis Test (p ≤ 0.055) |
| Acid-phosphatase (mM p-nitrophenol released/min/mg of protein) | 0.86±0.02 | 0.84±0.04 | 0.73±0.10 | 0.60±0.11 | Tukeys Test (p ≤ 0.137) |
| Alkaline-phosphatase (mM p-nitrophenol released/min/mg of protein) | 0.42±0.02 | 0.30±0.03 | 0.71±0.06 | 0.14±0.04* | Kruskal-Wallis Test (p ≤ 0.013) |
| Glutathione-S-Transferase (μM of GS-CDNB conjugated/min/mg of protein) | 0.219±0.002 | 0.365±0.003 | 0.333±0.001 | 0.376±0.009 | Kruskal-Wallis Test (p ≤ 0.027) |
* Significant from control