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. 2022 Aug 19;17(8):e0273385. doi: 10.1371/journal.pone.0273385

Fig 8. Urinary volume in 24 h per 100 g BM.

Fig 8

Urine samples were collected between 90 and 91 days, except for Ang-(3–4)-treated rats, which were collected between 91 and 92 days. (A) Responses to Losartan. Comparison of the urinary volume of CTRL and RBD rats without chronic administration of Losartan, and effects of Losartan administration, as indicated on the abscissa. (B) Responses to Ang-(3–4). Effects of oral administration of a single dose of Ang-(3–4) on the urinary volume of CTRL and RBD rats, as indicated on the abscissa. (C) Responses to Ang-(3–4) in rats previously treated with Losartan. Effects of oral administration of a single dose of Ang-(3–4) on the urinary volume of CTRL and RBD rats previously treated with Losartan, as indicated on the abscissa. (D) Effects of combined Losartan+Ang-(3–4) administration. Comparison of urinary volume between untreated CTRL and RBD rats vs. CTRL and RBD rats that were chronically given Losartan and a single dose of Ang-(3–4), as indicated on the abscissa. Bars are mean ± SEM; n = 5 (CTRL), n = 8 (RBD), n = 5 (CTRL+Los), n = 6 (RBD+Los), n = 5 (CTRL+Ang-(3–4)), n = 8 (RBD+Ang-(3–4)), n = 5 (CTRL+Los+Ang-(3–4)), n = 6 (RBD+Los+Ang-(3–4)). Differences between means were analyzed using one-way ANOVA followed by Bonferroni’s test for selected pairs. P values are indicated within the panels.