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. 2022 Aug 19;8(33):eabo4363. doi: 10.1126/sciadv.abo4363

Fig. 3. Z583 selectively inhibited the JAK3-dependent signaling pathway in hPBMCs.

Fig. 3.

(A) Four JAK subtypes (JAK1, JAK2, JAK3, and Tyk2) relay intracellular signal transduction with combinations of heterodimers or homodimers. (B) JAKs and STATs engage associated cytokines to mediate biological function. hPBMCs or CD34+ cells (for EPO) with or without various concentrations of Z583 were stimulated with JAK3-particular γc cytokines (C and E) or other cytokines (D and F). Western blots were carried out to evaluate the expression of cytokine-associated STAT phosphorylation. All data are from three independent experiments. Data are presented as means ± SEM. ##P < 0.01, ###P < 0.001 versus control group and *P < 0.05, **P < 0.01, and ***P < 0.001 versus alone cytokine-stimulating group. ns, no statistical significance.