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. 2022 Aug 19;8(33):eabo4363. doi: 10.1126/sciadv.abo4363

Fig. 4. Z583 inhibited the phenotypic maturation of DCs in vitro.

Fig. 4.

(A) Representative morphology of DC population after different treatment as shown by phase-contrast microscopy. iDCs from monocytes of C57BL/6 mice were pretreated with Z583 (10, 1, and 0.1 μM) for 1 hour and then followed by LPS stimulation for another 24 hours; percentage of CD80+CD11c+ (B) and CD86+CD11c+ (C) cells were analyzed by flow cytometry. The production of proinflammatory cytokines TNF-α (D), IL-6 (E), and IL-12 (F) in the supernatant was measured by enzyme-linked immunosorbent assay (ELISA). All data are from three independent experiments. Data are presented as means ± SEM; *P < 0.05, **P < 0.01, and ***P < 0.001 versus LPS-treated control group.