Fig. 5. Selective JAK3 inhibition with Z583 suppressed the naïve T cell proliferation and differentiation.

Naïve CD4⁺ T cells from lymph nodes of C57BC/6 mice were isolated to perform the proliferation and differentiation assays. (A) Naïve CD4⁺ T cells were stimulated with anti-CD3/anti-CD28; the effect of Z583 on T cell proliferation was analyzed by flow cytometry. CD4⁺ T cells were stimulated with anti-CD3/anti-CD28 in the presence or absence of Z583 (10, 1, and 0.1 μM) for 72 hours; the expression of IFN-γ (B) and T_H17A (D) was analyzed by flow cytometry. The production of IFN-γ (C) and T_H17A (E) by CD4⁺ T cells in the supernatant was measured by ELISA; the IC₅₀ values of Z583 (0.01, 0.3, 1, 3, and 10 μM) for inhibiting T cell differentiation were calculated. All data are from three independent experiments. Data are presented as means ± SEM; **P < 0.01 and ***P < 0.001 versus anti-CD3/anti-CD28–treated control group.